Document 0072
 DOCN  M9490072
 TI    Analysis of murine antibody responses to baculovirus-expressed human
       immunodeficiency virus type 1 envelope glycoproteins.
 DT    9411
 AU    Bristow RG; Douglas AR; Skehel JJ; Daniels RS; Virology Division,
       National Institute for Medical Research, Mill; Hill, London, U.K.
 SO    J Gen Virol. 1994 Aug;75 ( Pt 8):2089-95. Unique Identifier : AIDSLINE
       MED/94322004
 AB    An analysis of the humoral immunogenicity of a candidate AIDS vaccine
       (VaxSyn) in a murine model system is presented. Sera taken from a panel
       of mice immunized with the immunogen were analysed for their ability to
       bind a panel of gp120-representing peptides and limited reactivity to
       known sites of immunological interest was observed. Monoclonal
       antibodies (MAbs) were characterized as binding to a more restricted
       variety of regions on gp120 including C1, V2, V4 and the C terminus. A
       tetrazolium-based cytocidicity assay was used and shown to be an
       effective and objective method for the screening of human
       immunodeficiency virus (HIV)-neutralizing activity in large numbers of
       samples. None of the MAbs characterized in this study neutralize HIV-1
       reference strains. The significance of these findings in view of
       previous publications is discussed.
 DE    Animal  Antibodies, Monoclonal/BIOSYNTHESIS  Antigenic Determinants
       AIDS Vaccines/*IMMUNOLOGY  Baculoviridae/GENETICS  HIV/*IMMUNOLOGY  HIV
       Antibodies/*BIOSYNTHESIS  Mice  Mice, Inbred BALB C  Neutralization
       Tests/METHODS  Peptide Fragments/IMMUNOLOGY  Recombinant
       Proteins/IMMUNOLOGY  Sialoglycoproteins/GENETICS/IMMUNOLOGY  Support,
       Non-U.S. Gov't  Vaccines, Synthetic/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).